Chemical equipment such as buckets and test tubes contain liquid samples for examination. Cuvettes are used in spectroscopic analysis, while test tubes are used in common laboratory applications. The main difference between a bucket and a test tube is that a bucket has two straight sides, while a test tube does not. For example, a cell, a capillary, a quartz cell, a spectrophotometer bucket, etc. There are two sides where the light beam passes through in a bucket. Light scattering can be caused by scratches on these surfaces, resulting in inaccuracies in the final product.
You should make your spectrophotometer “zero” before using it so that all of your absorption measurements can have a baseline to compare with. Remove the empty space and place the experimental sample on the device. Slide the bucket into the designated slot and make sure it stays upright. Wait about 10 seconds until the needle is stable or until the digital numbers stop changing.
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For example, if you leave the spectrophotometer blank and then analyze only some of the samples and leave them blank again, the remaining samples will be inaccurate. When you remove the empty space, the calibration remains in place. When you measure the rest of your samples, the absorption of the white space is automatically subtracted. Before placing the bucket on the spectrophotometer, make sure it is as clean as possible to avoid interference from dirt or dust particles. With a lint-free cloth, remove any drops of water or dust on the outside of the bucket.